MAP2K1 (Human) Recombinant Protein
产品名称: MAP2K1 (Human) Recombinant Protein
英文名称: MAP2K1 (Human) Recombinant Protein
产品编号: P5676
产品价格: 0
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围: null
亚诺法生技股份有限公司(Abnova)
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- 所在区域 : 台湾
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- Specification
- Product Description:
- Human MAP2K1 (NP_002746.1, 1 a.a. - 393 a.a.) full-length recombinant protein with GST tag expressed in Escherichia coli.
- Theoretical MW (kDa):
- 70
- Form:
- Liquid
- Preparation Method:
- Escherichia coli expression system
- Purification:
- Glutathione sepharose chromatography
- Purity:
- 56 % by SDS-PAGE/CBB staining
- Activity:
- The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione- coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG. Substrate: Erk2. ATP: 100 uM.
- Storage Buffer:
- In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 5 mM DTT, 10% glycerol)
- Storage Instruction:
- Store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Loading 1 ug protein in SDS-PAGE
- Note:
- Result of activity analysis
-
- Application Image
- Functional Study
- SDS-PAGE
- Entrez GeneID:
- 5604
- Protein Accession#:
- NP_002746.1
- Gene Name:
- MAP2K1
- Gene Alias:
- MAPKK1,MEK1,MKK1,PRKMK1
- Gene Description:
- mitogen-activated protein kinase kinase 1
- Omim ID:
- 176872
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq
- Other Designations:
- protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
- Gene Pathway
- Acute myeloid leukemia
- B cell receptor signaling pathway
- Bladder cancer
- Chemokine signaling pathway
- Chronic myeloid leukemia
- Colorectal cancer
- Dorso-ventral axis formation
- Endometrial cancer
- ErbB signaling pathway
- Fc epsilon RI signaling pathway
- Fc gamma R-mediated phagocytosis
- Focal adhesion
- Gap junction
- Glioma
- GnRH signaling pathway
- Insulin signaling pathway
- Long-term depression
- Long-term potentiation
- MAPK signaling pathway
- Melanogenesis
- Melanoma
- Natural killer cell mediated cytotoxicity
- Neurotrophin signaling pathway
- Non-small cell lung cancer
- Pancreatic cancer
- Pathways in cancer
- Prion diseases
- Prostate cancer
- Regulation of actin cytoskeleton
- Renal cell carcinoma
- T cell receptor signaling pathway
- Thyroid cancer
- Toll-like receptor signaling pathway
- Vascular smooth muscle contraction
- VEGF signaling pathway
- Related Disease
- Abnormalities, Multiple
- Adenocarcinoma
- Carcinoma, Non-Small-Cell Lung
- Carcinoma, Squamous Cell
- Cognition Disorders
- Developmental Disabilities
- Ectodermal Dysplasia
- Genetic Predisposition to Disease
- Glioma
- Heart Defects, Congenital
- LEOPARD Syndrome
- Lung Neoplasms
- Mental Retardation
- Noonan Syndrome
- Pancreatic Neoplasms
- Skin Abnormalities
- Syndrome