MBD3 (C-term) Peptide
产品名称： MBD3 (C-term) Peptide
英文名称： MBD3 (C-term) Peptide
产品编号： P0169
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of MBD3.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to residues surrounding E279 of human MBD3.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Ammonium sulfate precipitation
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Biphasic kinetics of the human DNA repair protein MED1 (MBD4), a mismatch-specific DNA N-glycosylase.
Petronzelli F, Riccio A, Markham GD, Seeholzer SH, Stoerker J, Genuardi M, Yeung AT, Matsumoto Y, Bellacosa A.J Biol Chem. 2000 Oct 20;275(42):32422-9.
- 2.
- A mammalian protein with specific demethylase activity for mCpG DNA.
Bhattacharya SK, Ramchandani S, Cervoni N, Szyf M.Nature. 1999 Feb 18;397(6720):579-83.
- 3.
- Identification and characterization of a family of mammalian methyl-CpG binding proteins.
Hendrich B, Bird A.Mol Cell Biol. 1998 Nov;18(11):6538-47.
- Applications
- Western Blot (Transfected lysate)
- Western blot analysis of MBD3 (arrow) using rabbit MBD3 polyclonal antibody (Cat # PAB2214). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MBD3 gene (Lane 2) (Origene Technologies).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the MBD3 polyclonal antibody (Cat # PAB2214) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Entrez GeneID:
- 53615
- Protein Accession#:
- O95983
- Gene Name:
- MBD3
- Gene Alias:
- -
- Gene Description:
- methyl-CpG binding domain protein 3
- Omim ID:
- 603573
- Gene Ontology:
- Hyperlink
- Gene Summary:
- DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. [provided by RefSeq
- Other Designations:
- -