骨形成蛋白8B(BMP8B)重组蛋白

YB105Mu022
Recombinant Bone Morphogenetic Protein 8B (BMP8B)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 骨形成蛋白8B(BMP8B)重组蛋白[ PROPERTIES ]
Residues: Gly20~His399
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P55105
Host: E. coli
Subcellular Location: Secreted.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
 骨形成蛋白8B(BMP8B)重组蛋白0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 7.4
Predicted Molecular Mass: 46.7kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
 骨形成蛋白8B(BMP8B)重组蛋白[ SEQUENCES ]
The sequence of the target protein is listed below.
G GHLSHPPHVF PQRRLGVREP RDMQREIREV LG LPGRPRSR APVGAAQQPA
SAPLFMLDLY RAMTDDSGGG TPQPHLDRAD LIMSFVNIVE RDRTLGYQEP HWKEFHFDLT
QIPAGEAVTA AEFRIYKEPS THPLNTTLHI SMFEVVQEHS NRESDLFFLD LQTLRSGDEG
WLVLDITAAS DRWLLNHHKD LGLRLYVETE DGHSIDPGLA GLLGRQAPRS RQPFMVGFFR
ANQSPVRAPR TARPLKKKQL NQINQLPHSN KHLGILDDGH GSHGREVCRR HELYVSFRDL
GWLDSVIAPQ GYSAYYCAGE CIYPLNSCMN STNHATMQAL VHLMKPDIIP KVCCVPTELS
AISLLYYDRN NNVILRRERN MVVQACGCH
[ REFERENCES ]
1. Zhao G.Q., Hogan B.L. (1996) Mech. Dev. 57:159-168.
2. Zhao G.Q., et al. (1996) Genes Dev. 10:1657-1669.
3. Ying Y., et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98:7858-7862.
4. Whittle A.J., et al. (2012) Cell 149:871-885.