FITC标记的磷酸化活化T细胞核因子5蛋白抗体
产品名称： FITC标记的磷酸化活化T细胞核因子5蛋白抗体
英文名称： Anti-phospho-NFAT5 (Ser1197)/FITC
产品编号： HZ-9474R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
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Rabbit Anti-phospho-NFAT5 (Ser1197)/FITC Conjugated antibody
FITC标记的磷酸化活化T细胞核因子5蛋白抗体
| 产品编号 | bs-9474R-FITC |
| 英文名称 | Anti-phospho-NFAT5 (Ser1197)/FITC |
| 中文名称 | FITC标记的磷酸化活化T细胞核因子5蛋白抗体 |
| 别 名 | NFAT5 (phospho S1197); p-NFAT5(phospho S1197); Glutamine rich protein H65; KIAA0827; NF AT5; NF-AT5; NFAT 5; NFAT L1; NFAT like protein 1; NFAT5; NFAT5_HUMAN; NFATL 1; NFATL1; NFATZ; Nuclear factor of activated T cells 5; Nuclear factor of activated T cells 5 tonicity responsive; Nuclear factor of activated T cells; Nuclear factor of activated T-cells 5; OREBP; Osmotic response element binding protein; T cell transcription factor NFAT 5; T cell transcription factor NFAT5; T-cell transcription factor NFAT5; TonE binding protein; TonE-binding protein ; TonEBP; Tonicity responsive enhancer binding protein; Tonicity-responsive enhancer-binding protein. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 心血管 转录调节因子 淋巴细胞 t-淋巴细胞 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 166kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human NFAT5 around the phosphorylation site of Ser1197 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Members of the NFAT (nuclear factor of activated T cells) family of transcription factors are related to NFkB/Rel proteins and form cooperative complexes with the AP-1 proteins, Fos and Jun, on DNA to regulate cytokine expression in T cells. NFAT proteins are widely expressed and alternatively modified to generate splice variants, and they are localized to both the cytosol (NFATc) and to the nucleus (NFATn). NFAT1, NFAT2, and NFAT4 are predominantly expressed in immune cells, and NFAT2 and NFAT3 are expressed at high levels in cardiac tissues. In addition to activating cytokine gene transcription, NFAT2 is also implicated in cardiac valve development, and NFAT3 is involved in cardiac hypertrophy. NFAT5 is detected in both immune and nonimmune cells and, like other NFAT proteins, contains a highly conserved Rel-like binding domain that mediates NFAT proteins associating with specific consensus sequences on DNA. NFAT proteins are activated by increases in intracellular calcium, which leads to the calmodulin-dependent phosphatase, calcineurin, dephosphorylating NFAT proteins. This activating event induces a conformational change in the protein structure that exposes the nuclear localization signal and facilitates the translocation of NFAT proteins from the cytosol into the nucleus. Function: Plays a role in the inducible expression of genes.Regulates hypertonicity-induced cellular accumulation of osmolytes. Subunit: Homodimer when bound to DNA, completely encircles its DNAtarget. Does not bind with Fos and Jun transcription factors. Subcellular Location: Nucleus. Tissue Specificity: Highest levels in skeletal muscle, brain, heart and peripheral blood leukocytes. Also expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Post-translational modifications: Phosphorylated at Thr-135 by CDK5 in response to osmoticstress; this phosphorylation mediates its rapid nuclearlocalization. Similarity: Contains 1 RHD (Rel-like) domain. Database links: Entrez Gene: 10725 Human Entrez Gene: 54446 Mouse Entrez Gene: 307820 Rat Omim: 604708 Human SwissProt: O94916 Human SwissProt: Q9WV30 Mouse Unigene: 371987 Human Unigene: 390057 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
NFK/ReR蛋白家族成员的NFAT（活化T细胞核因子）家族成员与NFKB/RE蛋白相关，并与AP-1蛋白、FOS和Jun形成协同配合物，以调节T细胞中的细胞因子表达。NFAT蛋白被广泛表达并选择性地修饰以产生剪接变异体，并且它们定位于胞质溶胶（NFATC）和细胞核（NFATN）。NFAT1、NFAT2和NFAT4主要在免疫细胞中表达，NFAT2和NFAT3在心肌组织中高表达。除了激活细胞因子基因转录外，NFAT2也参与了心脏瓣膜的发育，NFAT3参与了心肌肥大。NFAT5在免疫和非免疫细胞中都被检测到，和其他NFAT蛋白一样，包含一个高度保守的类受体结合域，介导NFAT蛋白与DNA上的特异性一致序列相关。NFAT蛋白通过细胞内钙的增加而激活，这导致钙调素依赖性磷酸酶、钙调神经磷酸酶、去磷酸化NFAT蛋白。这种激活事件诱导蛋白质结构中的构象变化，暴露细胞核定位信号并促进NFAT蛋白从细胞质转运到细胞核。